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920-177 – Nortel Contact Center Rls. 7.0 Administration and Scripting Questions and Answers by Killexams.com

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Nortel Scripting test contents

brief and long-read genome sequencing methodologies for somatic variant detection; genomic analysis of a patient with diffuse gigantic B-mobilephone lymphoma | 920-177 Free test PDF and Latest Topics

patients and ethics

The country wide health provider (NHS) health analysis Authority South central—Oxford C research Ethics Committee permitted this examine and all research changed into carried out according to principal laws and instructions. Written counseled consent became acquired from the patient in response to current Oxford Radcliffe Biobank (ORB) guidelines (Oxford C research Ethics Committee No: 09/H0606/5+5). Consent turned into for evaluation of tumor and constitutional DNA. feedback of clinically actionable germline variants changed into not obligatory.

The Oxford Radcliffe Biobank is a analysis facility of the institution of Oxford and the Oxford Radcliffe foundation Hospitals have confidence and offers governance, administration and regulatory oversight for approved analysis initiatives.sample guidance and genome sequencing Tumor tissue managing

The patient underwent biopsy of simple and/or metastatic cancer to achieve clean tissue for sequencing (as up to now described in10). in brief, fresh tissue samples were amassed on the time of resection of the ileocaecal and regional lymph nodes. The histopathology document established DLBCL (ABC class). Samples were snap-frozen in liquid nitrogen and an H&E frozen histology section turned into taken to confirm tumour content material. only samples with microscopically estimated tumor mobile content material of > 40% had been used for sequencing. Frozen tissue was thawed rapidly for nucleic acid extraction and sequencing.DNA extraction and short-examine complete genome sequencing

Tumor DNA became extracted from fresh frozen tissue the usage of the All Prep Mini DNA Extraction package (QIAGEN), as described within the company’s protocol. Germline DNA become remoted from 1.5 ml peripheral blood the use of the QIASymphony DSP DNA Midi package (QIAGEN), based on the manufacturer’s protocol. Libraries of fragments with a goal size of 350 bp were generated from 1 µg sheared genomic DNA the usage of the TruSeq PCR-Free library instruction package (Illumina, San Diego, CA). 2 × one hundred fifty paired-conclusion sequencing turned into carried out using the HiSeq4000. (Illumina, San Diego, CA). WGS become performed at a deliberate coverage of 30 × for the germline DNA and of seventy five × for the tumour.long-study library coaching and sequencing: tumour DNA

2 µg of lymphoma DNA turned into thawed fragmented in forty nine µl of Nuclease Free Water (NFW) for 2 min at 7,000 revolutions per minute (rpm) using g-TUBE (Covaris, Woburn, MA, united states of america) following brand strategies. FFPE restoration, end fix-dA tailing and PCR Adapter (PCA) ligation, were carried out following Oxford Nanopore applied sciences’ (ONT) 1D Low input genomic DNA with PCR (SQK-LSK108) edition LIP_9021_v108_revL_11Nov2016. conclusion restore incubation times were prolonged to 30 min at 20 °C and 30 min at 65 °C. Incubation of DNA with Agencourt AMPure XP beads (Backman Coulter Inc., Brea, CA, united states of america) and elution instances had been increased to 20 and 10 min at room temperature respectively. Pre-PCR measurement option was carried out as follows: After washing the beads with 70% ethanol, the PCA ligated DNA turned into re-suspended in one hundred µl of NFW and transferred to a brand new 1.5 µl Lobind Tube (Eppendorf, Hamburg, Germany). heading off pelleting the beads on magnetic bar, an further quantity of forty µl of beads became delivered to the old re-suspended cloth ensuing into a 0.4X ratio of beads to DNA volume. The tube was incubated on the Hula Mixer (Thermo Fisher Scientific, Waltham, MA, usa) for 20 min at room temperature. The beads have been washed twice with 200 µl of 70% ethanol and re-suspended in 28 µl of NFW followed by using 10 min elution at room temperature. The PCA ligated and measurement selected DNA become diluted to a attention of 10 ng/µl in NFW. The PCR reaction become install as follows: forty six μl Nuclease-free water, 2 μl Primers (PRM, Oxford Nanopore technologies, Oxford, UK), 2 μl 10 ng/μl adapter ligated template, 50 μl LongAmp Taq 2 × grasp combine (New England Biolabs, Ipswich, MA, u . s . a .). initial denaturation became for 3 min at ninety five °C, denaturation 15 secs at ninety five °C (15 cycles), annealing 15 secs at sixty two °C (15 cycles), extension 15 min at sixty five °C (15 cycles), closing extension 15 min at sixty five °C, hang at 4 °C. PCR products had been cleaned up with a 0.4X ratio of beads to DNA quantity and eluted in 25 µl NFW. five aliquots of 1.5–2 µg size selected and amplified tumour DNA have been organized and sequenced in response to the ONT protocol kit 9 chemistry version GDLE_9056_v109_revC_02Feb2018. These were run on 4 PromethION circulation cells the use of the MinKNOW utility version 1.18.02 for sixty four h.lengthy-read library preparation and sequencing: germline DNA

2 µg of germline DNA have been handled following the identical pre-PCR methods as per the tumour pattern above. put up-PCR techniques had been accomplished in response to a newly up-to-date edition of the ONT SQK-LSK109 package protocol, GDE_9063_v109_revC_04Jun2018. five size chosen and amplified germline DNA aliquots of 1–1.5 µg had been run on four PromethION movement cells the usage of ONT MinKNOW utility version 1.18.02 for 64 h.

In parallel, two further R9.four.1 MinION flow cells were run the usage of 1 µg of measurement selected and amplified germline and tumour DNA following the SQK-LSK109 protocol version GDE_9063_v109_revA_23May2018 (Oxford Nanopore technologies, Oxford, UK).RNA lymphoma cDNA library preparation and sequencing

RNA become extracted the usage of the AllPrep Extraction equipment (Qiagen). The lymphoma transcriptome become processed in response to the Oxford Nanopore SQK-PCS108 cDNA-PCR sequencing protocol (edition PCS_9035_v108_revD_26Jun2017, final update 25/10/2017). They used 500 ng of total RNA into a final extent of 9 µl. observe that because of the low enter of the long-established clinical aliquot, they could not recuperate the most advantageous beginning input of ~ 50 ng of PolyA RNA. The mRNA turned into centered for reverse transcription the use of Nanopore oligo-dTs. consequently, full-size double stranded cDNA turned into generated employing the Nanopore Strand Switching Primer and amplified in 50 µl response volumes.

The PCR response contained 5 µl cDNA, 25 µl 2X LongAmp Taq master combine (NEB Biolabs Inc, Ipswich, Massachusetts, u . s ., cat. #M0287S), 1.5 µl Nanopore cDNA Primers and 18.5 µl NFW. The reactions were incubated at 95 °C for 30 s, adopted through 15 cycles of (ninety five °C for 15 s, 62 °C for 15 s, sixty five °C for eight min) with a ultimate extension at sixty five °C for six min. The amplification products have been normalised to ~ 400 fmol into 20 µl of Nanopore quick Annealing Buffer. The cDNA changed into adapter ligated including 5 µl of Nanopore cDNA Adapter mix for 5 min at room temperature. The adapter ligated library turned into subsequently cleaned-up via adding forty µl AMPure XP beads, incubating for 5 min at room temperature and re-suspending the pellet twice in a hundred and forty µl of Nanopore Adapter Binding Beads. The purified ligated cDNA was then eluted in 12 µl of Nanopore Elution Buffer. The library changed into run on a MinION R9.4.1 circulate cellphone the usage of the 48 h PCS108 MinION sequencing script.Bioinformatic analysis of the Illumina data

analysis changed into performed the use of a bespoke, locked-down, and edition-controlled bioinformatics pipeline based on the required specification for clinically accredited laboratories.

Paired-conclusion alignment of sequencing records in opposition t the reference genome hg19 (GRCh 37) turned into performed using the entire-Genome Sequencing utility v2.0, in line with Isaac Alignment device, within BaseSpace (Illumina). Somatic single nucleotide (SNV) and insertion/deletion (InDel) variant calling analysis became carried out using the Tumour-normal utility v2.0, based on Strelka, within BaseSpace. Calls were annotated the usage of variant impact predictor (VEP) within Ensembl-equipment v84, together with COSMIC v71.

Manta (v0.23.1, as part of Illumina’s Tumor standard application v2.0.0) changed into run for somatic structural variant calling.Detection of obtained CN pursuits

WGS bam information (hg19) have been analysed for reproduction quantity (CN) routine using ngCGH (https://github.com/seandavi/ngCGH) for the paired tumour:germline analysis and ngbin (BioDiscovery, Inc., El Segundo, California, united states of america, obtainable from http://www.biodiscovery.com) for single pattern analyses. A window measurement of 300 reads become applied for ngCGH, and a window dimension of 1 kb and skim depth of ten for ngbin. B-allele frequency assistance was received from vcf information generated using Platypus68. Singleton and matched tumour-germline Log2R outputs had been visualised together with B-allele frequency information the use of Nexus Discovery edition 10.1 (BioDiscovery, Inc., El Segundo, California, united states). CN routine and areas of homozygosity (ROHs) were flagged the usage of the SNP Rank Segmentation surroundings. All putative pursuits had been visualised and curated manually prior to filtering in Microsoft Excel to get rid of germline copy number versions (CNVs), germline ROHs and calls due to underlying sequence complexity (eg. segmental duplications, tandem repeat regions) or insufficient self belief on visualisation. For bought ROHs, called replica neutral lack of homozygosity (cnLOH), the reporting size threshold changed into > 2 Mb. All acquired reproduction quantity events have been go-checked the use of IGV, hunting for helping proof from examine pairs mapping to the breakpoint regions. The last Microsoft Excel file covered all filtered hobbies intersected with both database37 and ‘in condominium’ gene lists comprising common genes of hobby in melanoma.Bioinformatic evaluation of the Nanopore statistics

Nanopore reads had been basecalled with Albacore v 2.3.1 or, for later runs, guppy v 1.6.0 (Oxford Nanopore applied sciences; be aware guppy 1.6.0 contained the same neural community model to Albacore 2.3.1 however became optimised to run on GPUs). They have been then trimmed with Porechop v 0.2.369 and mapped using minimap2 v 2.1043. Trimmed reads were moreover mapped with ngmlr v 0.2.7, considering that the developers of this mapper had pronounced superior outcomes for structural variant calling42.Somatic SNV calling

Reads mapping to chromosome 17 (with minimap2) were selected and split into mapped sections of max 100 bp in length. FreeBayes v 1.0.238 changed into run on the split bams, for tumour and germline samples one at a time, the use of the contamination parameters RH/RA = 0.7/0.1. These parameters had produced respectable effects for variant calling devoid of prior phasing in previous work39. Indels and MNPs have been now not called. each tumour and germline bams have been filtered in response to here standards: QUAL > 1 & NUMALT < 2 & SAF > 1 & SAR > 1. After this initial filtering, germline calls have been subtracted from tumour calls in the event that they shared the CHROM, POS and ALT fields within the vcf. Having up to now followed that strand bias is regularly an indicator of a repeated Nanopore error, they eliminated calls the place the fraction of alternate allele observations become more than 4 × bigger or decrease within the ahead strand vs the reverse strand. The ensuing somatic vcf turned into examined for overlap with the brief-examine SNV calls and the distributions of here residences have been plotted: AO (alternate allele observations), RO (reference allele observations), QA (alternate allele fine sum in phred), QR (reference allele first-rate sum in phred), MQM (imply mapping first-class of accompanied alternate alleles), AB (the ratio of reads displaying the reference allele to all reads), SAP (strand balance likelihood for the alternate allele), as shown in Supplementary Fig. S6. in accordance with these distributions right here criteria have been used for remaining filtering: AO > 10 & RO > 10 & QA > a hundred & QR > 100 & AO < a hundred & RO < a hundred & SAP < 30 & MQM > 55. The filtering criteria selected here have been utilized to the chromosome 22 facts.Somatic SV calling

Sniffles v1.0.942 turned into used to name structural variations with the following parameters: -l one hundred -s 10 –genotype. Germline calls were subtracted from tumour calls in response to matching ALT fields (i.e. SV type), and each the starts and ends being inside (germline SV length)/100 bases of every different. remaining somatic calls were then filtered in response to SVLEN > 10,000 & !(CHR2 = hs37d5) & actual. IMPRECISE calls with AF > 0.2 (as suggested through Sniffles) had been examined one at a time. SV calling became carried out for reads mapped with minimap2 and reads mapped with ngmlr. They examined all lengthy-study and short-study SV calls visually within the Integrative Genomics Viewer (IGV)70, with tracks of long-examine records, short-examine facts, and segmental duplications simultaneously loaded. in this method every name was classfied as either genuine or false depending on no matter if it turned into deemed to be a genuine somatic abnormality, or a germline variant or mapping artefact.comparison of brief-read and lengthy-read SV calls

Lists of calls from the three different strategies (Illumina + Manta, Nanopore + minimap2 + Sniffles, Nanopore + ngmlr + Sniffles) were examined to come to a decision where the same SV became reported by means of diverse methods (see Supplementary desk S3). The breakpoints of each entertaining call had been looked at in IGV to assess no matter if there changed into adequate facts to class a call as a genuine somatic variant. If there turned into facts of reads aiding the breakpoint in the germline sample, the call become classed as ‘false’. False discovery prices and false poor charges they calculated by means of assuming that all proper editions have been picked up by means of one of the three methods, and then the usage of right here formulae (TP = authentic nice; FP = false advantageous):

$$ \beginaligned \textual contentFNR & = \left( \textual contentvariety of TPs not detected with this formula \right)/\left( \texttotal TPs \correct) \\ \textFDR & = \left( \textvariety of FPs detected with this formulation \right)/\left( \textual contenttotal calls from this formulation \correct) \\ \conclusionaligned $$Detection of got CN activities

Aligned reads were split into aligned sections of max a hundred bp in size, as described for SNV calling above. The number of reads with MAPQ >  = 20 within a hundred kb home windows became counted, for both the tumour and germline bam files. Let N(w) be the read count in the germline demo for window, w, and T be described in a similar way for the tumour pattern. The normalised log study count number ratio, R, changed into calculated as

$$ R\left( w \right) = \left\{ \startarray*20ll \log_2 \fracT\left( w \appropriate)N\left( w \appropriate) – \log_2 \frac252187, &\quad N\left( w \right) > \textual contentimply\left( N \right) – 2 \times \textsd\left( N \correct) \\ \textual contentNA, &\quad \textotherwise. \\ \conclusionarray \correct. $$

The R package DNAcopy71, became used to operate circular binary segmentation and detect change elements the usage of the following parameters: alpha = 0.01, min.width = 5, undo.splits = ”sdundo”, undo.SD = three. The mean price of aspects in all segments longer than 500 windows and representing ploidy = 2 (i.e. clustered round 0) changed into calculated as 0.094. This turned into subtracted from all segment skill, to account for the ploidy of the tumour demo being improved than 2. The purity of the tumour demo changed into then calculated by way of in a similar way computing the imply cost of facets in segments representing ploidy = 1, three, four (identifiable as distinct clusters of phase skill). This gave three separate estimates of purity – 0.sixty one, 0.sixty six and 0.sixty four, the place purity was described because the volume of tumour DNA within the tumour pattern as a proportion of total DNA (tumour + germline). A purity cost of 0.63 became used to calculate the theoretical section capacity for values of ploidy from 1 to 6 and then classify the recorded phase means consequently, for both the autosomes (with ploidy 2 in the germline) and the sex chromosomes (with ploidy 1 in the germline).Estimating anticipated frequencies of the p.Arg249Met somatic variant in TP53

For this calculation they rounded the purity estimate to 60%. therefore they count on that forty% of cells on regular include germline genetic cloth. Let the fraction of cells containing the somatic deletion encompassing TP53 be \(x\) %, then the fraction of tumour cells with out the deletion is (60—\(x\))%.

If the somatic SNV is in cis with the deletion, the fraction of reads containing the somatic SNV will also be estimated as at most:

$$ \beginaligned & \frac\# \text tumour cells with out the deletion 2 \instances \left( \# \text cells with out the deletion \right) + \# \text cells with deletion \\ & \quad = 60 – x/2 \times \left( 60 – x + forty \appropriate) + x \\ & \quad = 60 – x/200 – x \\ \endaligned $$

Which takes a highest cost of 30% when x = 0. If the somatic SNV is in trans with the deletion, the fraction of reads containing the somatic SNV may also be estimated as at most:

$$ \beginaligned & \frac\# \textual content tumour cells 2 \times \left( \# \textual content cells devoid of the deletion \right) + \# \textual content cells with deletion \\ & \quad = 60/200 – x \\ \endaligned $$

This varies from 30 to forty three% as \(x\) varies from 0 to 60.Phasing

a listing of heterozygous SNVs was obtained by working Illumina’s Isaac variant caller on the germline bam. whatshap phase changed into then run using this vcf and Nanopore reads from both the germline and tumour samples. The ensuing phased SNV calls have been used together with whatshap haplotag to assign haplotype and phase set tags to Nanopore reads from the tumour sample. Haplotag bams were visualised in IGV, grouped by using haplotype and coloured by means of section set.
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